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General laboratory animal guidelines include:
  • Too much blood collected at any one time may cause hypovolemic shock, physiological stress and even death. If smaller volumes are collected too frequently, anemia may result.
  • As a general rule, 10% of the total blood volume can be collected at one time every 2-4 weeks. Or 1% at intervals of 24 hours or more. Total blood volume can be calculated as approximately 7.5% of body weight.
  • The estimated volume at exsanguination is approximately half of the total blood volume.


Average Adult Weight Total Blood Volume Maximum Sample in mls 2-4 Weeks Estimated Exsanguination Volume
6 kg 450 ml 45 ml 225 ml

  • If you are not experienced in blood collection techniques, trainingtraining is available.

Collection Site Advantages Disadvantages
Femoral Vein
  • Maximum volume of blood can be collected
  • Training is required
  • Anesthesia required
Femoral Artery
  • Maximum volume of blood can be collected
  • Training is required
  • Anesthesia required
Saphenous Vein
  • Collection of small to moderate blood sample only
  • Training is required
  • Anesthesia required